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Polygonatum Kingianum Genome Size Estimation by Flow Cytometry
Xiao-Lei Chen,
Kun Cong,
Zhe Xu,
Hua-Li Qian,
Hao Chen,
Jian-Yun Su,
Lei Zhang,
Jia-Hong Dong,
Peng-Zhang Ji
Issue:
Volume 10, Issue 6, December 2022
Pages:
203-208
Received:
7 October 2022
Accepted:
1 November 2022
Published:
4 November 2022
DOI:
10.11648/j.jps.20221006.11
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Abstract: Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding.
Abstract: Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collect...
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Release and Registration of “Miju” Bread Wheat (Triticum aestivum L.) Variety
Aliyi Kedir,
Seyoum Alemu,
Kuma Kebede
Issue:
Volume 10, Issue 6, December 2022
Pages:
209-213
Received:
4 October 2022
Accepted:
4 November 2022
Published:
14 November 2022
DOI:
10.11648/j.jps.20221006.12
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Abstract: Improved varieties play an important role in enhancing production and productivity of a given crops and there by contributing to the change farmers’ livelihood and other organs those engaged with crop production. But, there are different agro-ecologies in Ethiopia, that challenging continues production of crops and crops respond differently to this different range of agro-ecologies. Therefore, there is the need to get high yielding and stable improved varieties across different location by conducting multi-location or multi-environmental trails. By considering this condition, this research experimental activity was conducted at three different locations (Bore, Abayi and AnaSora) for two consecutive years 2020 and 2021 main cropping seasons. Randomized complete block design with three replications was used as experimental design. Twenty (20) bread wheat genotypes included as testing materials and were evaluated against two standard checks (Danda’a and Hidase). The experimental unit had 2.5 m length by 1.2m width was used for this trial with an area of 3m2. The best genotype that gave high grain yield and as the same time stable across locations was selected. This newly selected and released genotype with the pedigree of CHEN/AEGILOPSSQUARROSA (TAUS (//BCN/3/BAV92/4BERKUT, was named as MIJU officially. This variety was developed by bore agricultural research center, Oromia Agricultural Research Institute. AMMI and GGE biplot analysis, revealed that genotype ETBW7082 (MIJU) is stable and high yielding (5.9 ton/ha) with a yield advantage of 35% over the best standard check Hidase. Thus, this new variety, MIJU was released in 2022 after decision committees of ministry of agriculture plant variety release, protection and seed quality control directorate officially and recommended for wider productions.
Abstract: Improved varieties play an important role in enhancing production and productivity of a given crops and there by contributing to the change farmers’ livelihood and other organs those engaged with crop production. But, there are different agro-ecologies in Ethiopia, that challenging continues production of crops and crops respond differently to this...
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Identification of Three Chinese Herbal Medicines and Their Adulterants Based on Allele-Specific Diagnostic PCR
Hao Chen,
Xiao-Lei Chen,
Jian-Yun Su,
Yan-Ying Li,
Xiao-Bin Hu,
Zhe Xu,
Hua-Li Qian,
Lei Zhang,
Jia-Hong Dong,
Peng-Zhang Ji
Issue:
Volume 10, Issue 6, December 2022
Pages:
214-221
Received:
14 October 2022
Accepted:
19 November 2022
Published:
29 November 2022
DOI:
10.11648/j.jps.20221006.13
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Abstract: Owing to the high pharmacological and economic value, the Chinese medicinal herbs: Paris polyphylla var. yunnanensis, Polygonatum kingianum and Bletilla striata, whose quality has been damaged by their adulterants. In this study, the allele-specific diagnostic PCR method was used to identify three herbal medicines and their adulterants. And the nuclear gene ITS2 sequence and chloroplast gene psbA-trnH sequence, as international common barcode sequences, are widely used in the molecular identification of medicinal plants. Therefore, three pair of specific PCR primers were respectively designed according to the sequence of Paris polyphylla var. yunnanensis on the ITS2, Polygonatum kingianum and Bletilla striata on the psbA-trnH, using Primer 5.0. And these specific identification primers could amplify special bands respectively at 130bp, 331bp and 508bp, while adulterants could not amplify the bands. The results showed that this method could respectively distinguish three herbal medicines and their adulterants. And after many experiments, the method had good repeatability. The allele-specific PCR identification method established in this study is easy to operate and fast in identification. This method can distinguish three kinds of traditional Chinese medicines and their adulterants, which provides a reference for the identification of other traditional Chinese medicines, so as to further protect the Chinese herbal medicine industry from economically motivated adulteration.
Abstract: Owing to the high pharmacological and economic value, the Chinese medicinal herbs: Paris polyphylla var. yunnanensis, Polygonatum kingianum and Bletilla striata, whose quality has been damaged by their adulterants. In this study, the allele-specific diagnostic PCR method was used to identify three herbal medicines and their adulterants. And the nuc...
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Visual Detection of Rf2 Event in Transgenic Rapeseed (Brassica napus L.) Using Loop-Mediated Isothermal Amplification
Wang Jiaying,
Zhao Lei,
Li Wen,
Huang Suwen,
Zhang Jihong
Issue:
Volume 10, Issue 6, December 2022
Pages:
222-226
Received:
19 October 2022
Accepted:
2 November 2022
Published:
29 November 2022
DOI:
10.11648/j.jps.20221006.14
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Abstract: Specific methods have been developed in order to efficiently detect GMOs (genetically modified organisms) in both feed and food. Current approaches often rely on thermal cycling devices such as PCR amplifier, which makes it difficult for applications in the wild. Therefore a visual LAMP (Loop-Mediated Isothermal Amplification) method for rapid screening Rf2 event in transgenic rapeseed (Brassica napus L.) is established in this paper. For primer design, position 167-366 in the right border junction sequence of B. napus trangenic line Rf2 (accession number: EU090197.1) was chosen as the amplicon. Reaction mixture (at the volume of 25 μL) consisted of 12.5 μL 2×buffer mix, 1.28 μM FIP, 1.28 μM BIP, 0.16 μM F3, 0.16 μM B3, 480 U/mL Bst DNA polymerase, 2.0 μL DNA template and 5.0 μL ddH2O. Dye (SYBR Green I) was pre-added onto the tube lid. The reaction tube was incubated at 60°C for 60 min, followed by heating at 80°C for 10 min to end it. To mix dye with reaction mixture, the tube was centrifuged for 60 s at 8,000 r/min. Corresponding analysis results indicate that this LAMP assay is highly specific and sensitive (1.15×103 copies/μL). In one word, the visual LAMP method specific for Rf2 event in rapeseed, which turns out to be simple, time-effective, sensitive without relying on expensive instruments, is suitable for quick screening in ports.
Abstract: Specific methods have been developed in order to efficiently detect GMOs (genetically modified organisms) in both feed and food. Current approaches often rely on thermal cycling devices such as PCR amplifier, which makes it difficult for applications in the wild. Therefore a visual LAMP (Loop-Mediated Isothermal Amplification) method for rapid scre...
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Potato (Solanum tuberosum L.) Microplants and Minitubers Effected by the Combination of Gibberellic Acid (GA3) and Indole 3 Acetic Acid (IAA)
Issue:
Volume 10, Issue 6, December 2022
Pages:
227-234
Received:
20 August 2022
Accepted:
13 September 2022
Published:
30 November 2022
DOI:
10.11648/j.jps.20221006.15
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Abstract: The purpose of this study, to find out the effect of GA3 and IAA on potato micro plants in vitro and further performance in net house and in field. This study was carried out between 2014-2015 in the Central Potato Research Institute Modipuram, Meerut. In this study used different concentrations of GA3 and IAA in combinations. For this experiment used 3 node stems cutting of potato variety Kufri Bahar and Kufri Surya with MS media supplemented with different concentrations and combinations of GA3+IAA (0.05 mg GA3/L+0.005 mg IAA/L, 0.1 mg GA3/L+0.01 mg IAA /L, 0.2 mg GA3/L+0.01 mg IAA /L, 0.1 mg GA3/L+0.02 mg IAA /L, 0.2 GA3 mg/L+0.02 mg IAA /L and 0.4 mgGA3/L+0.04 mg IAA/L) for microplant culture. Application of GA3+IAA at 0.4 mg/L+0.04 mg/L significantly increased length of shoot, number of nodes, internode length, number of leaves, length of root and fresh weight of shoot per plant in vitro. Similarly, the application of GA3+IAA at 0.4 mg/L+0.04 mg/L also increased the growth and yield parameters of potato variety Kufri Bahar and Kufri Surya in net house and in field. Potato quality and quantity can therefore be improved through application of 0.4 mgGA3/L+0.04 mg IAA/L.
Abstract: The purpose of this study, to find out the effect of GA3 and IAA on potato micro plants in vitro and further performance in net house and in field. This study was carried out between 2014-2015 in the Central Potato Research Institute Modipuram, Meerut. In this study used different concentrations of GA3 and IAA in combinations. For this experiment u...
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Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire
Guinagui N’Doua Bertrand,
Yeo Gnenakan,
Tuo Seydou,
Kouda Affiba Genevieve,
Lehi Malidy Irenee,
Danon Aubin Silvere Djiwha,
Fatogoma Sorho
Issue:
Volume 10, Issue 6, December 2022
Pages:
235-240
Received:
24 September 2022
Accepted:
28 October 2022
Published:
8 December 2022
DOI:
10.11648/j.jps.20221006.16
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Abstract: Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.
Abstract: Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly varia...
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