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Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of Rf1 Event in Transgenic Rapeseed (Brassica napus L.)
Zhang Jihong,
Wang Jiaying,
Zhao Lei,
Huang Suwen,
Chen Xianfeng
Issue:
Volume 10, Issue 3, June 2022
Pages:
86-90
Received:
1 April 2022
Accepted:
18 April 2022
Published:
7 May 2022
DOI:
10.11648/j.jps.20221003.11
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Abstract: Rapeseed (Brassica napus L.) is one of the most studied crops for genetic engineering. Up to now, at least 11 GM rapeseed varieties have been approved for commercial production. During this study, a simple and rapid LAMP method was established for detection of Rf1 event in transgenic rapeseed. Position 143-353 in the right border junction sequence of B. napus transgenic line Rf1 (accession number: EU090199.1) was chosen as the target region for primer design and LAMP detection. Reaction mixture at the volume of 25 μL contained 12.5 μL 2×buffer mix, 1.28 μM FIP, 1.28 μM BIP, 0.16 μM F3, 0.16 μM B3, 0.64 μM LF, 480 U/mL Bst DNA polymerase, 2.0 μL DNA template and 4.6 μL ddH2O. SYBR Green I was added to the tube lid. After incubation at 60°C for 60 min, the reaction was terminated by heating at 80°C for 10 min. Then the tube was centrifuged for 60 s at 8,000 r/min to mix dye with reaction mixture. Results indicate that our LAMP assay is highly specific and sensitive (9.2×103 copies/μL, 0.001%). This LAMP assay for Rf1 event in rapeseed, which is simple, time effective, specific, sensitive and result visible without expensive instruments, is suitable for application in wide fields.
Abstract: Rapeseed (Brassica napus L.) is one of the most studied crops for genetic engineering. Up to now, at least 11 GM rapeseed varieties have been approved for commercial production. During this study, a simple and rapid LAMP method was established for detection of Rf1 event in transgenic rapeseed. Position 143-353 in the right border junction sequence ...
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A Survey of Insect Pests Attacking Quinoa (Chenopodium quinoa) and Their Natural Enemies in Zimbabwe
Onias Dube,
Nhamo Mudada,
Tsvakai Gama,
Tavagwisa Muziri
Issue:
Volume 10, Issue 3, June 2022
Pages:
91-95
Received:
8 April 2022
Accepted:
26 April 2022
Published:
7 May 2022
DOI:
10.11648/j.jps.20221003.12
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Abstract: Quinoa (Chenopodium quinoa Wild.) was first introduced in Zimbabwe in 2017. As a new crop introduction, studies to understand the agronomic and biotic factors affecting quinoa cultivation are needed. A study to identify pests associated with quinoa production was therefore carried out at Midlands State University farm in Zimbabwe. A survey was conducted to determine the presence and populations of insect pests attacking quinoa crop during production. Field scouting of five (5) tagged plants per plot was done using canopy observation, buttressed with sticky and pitfall traps. Sampling was done when 50% of the crop had reached key physiological stages i.e early vegetative stage, vegetative stage, flowering, milk dough stage, dough stage, physiological maturity and at harvest. The most abundant pests observed were aphids, brown stink bugs, grasshoppers, and lepidopteron bollworms. Natural enemies observed were black ants, brown ants and ladybird beetles. Signs of insect damage observed include weakening of panicles, chlorosis of leaves, leaf eating and sooty mould development on the panicles. Findings of the study showed that quinoa attracts a diversity of leaf eating and sap sucking insects. This is the first study of insect pests associated with quinoa in Zimbabwe, and the results of the study indicate the need to develop insect pest management strategies for quinoa production in the country.
Abstract: Quinoa (Chenopodium quinoa Wild.) was first introduced in Zimbabwe in 2017. As a new crop introduction, studies to understand the agronomic and biotic factors affecting quinoa cultivation are needed. A study to identify pests associated with quinoa production was therefore carried out at Midlands State University farm in Zimbabwe. A survey was cond...
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Effect of NPS Fertilizer Rates on Yield and Yield Components of Sesame (Sesamum indicum L.) Varieties at Uke, Western Ethiopia
Kelemua Zebene,
Negash Geleta
Issue:
Volume 10, Issue 3, June 2022
Pages:
96-105
Received:
28 March 2022
Accepted:
28 April 2022
Published:
7 May 2022
DOI:
10.11648/j.jps.20221003.13
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Abstract: In Ethiopia, sesame is the leading oil crop followed by Niger seed and linseed; and oil seed is the third largest sector after cereal and pulse. Production and productivity of sesame is greatly affected by lack of optimum fertilizer rate, inappropriate sowing time, lack of optimum plant population, biotic and a biotic factor such as disease and pest infestation, low yielding variety, post-harvest lost, low fertilizer response, difference in capsule maturity, shattering etc. The study was carried out in 2018 main cropping season at Uke in Guto Gida District of East Wollega Zone of Oromia Regional State with the objectives of investigating the effects of NPS fertilizer rates on yield and yield components of sesame varieties. The treatment consisted of three sesame varieties (Chalsa, Dicho and Obsa) and five levels of NPS fertilizer (0, 50, 100, 150 and 200 kg/ha) and combined factorially and laid out in Randomized Complete Block Design with three replications. Data were taken for agronomic traits and subjected to analysis of variance using General Liner Model procedures of SAS (SAS, 2004) and means differences were tested for their significance using least significance difference method. The results of analysis of variance indicated that the effects of varieties were significant (P<0.05) for all parameters except for days to 50% emergency, length of capsule bearing zone and capsule length. Similarly, the effects of NPS fertilizer rates were significant (P<0.05) for all parameters. The interaction effect of varieties by NPS fertilizer rates were significant (P<0.05) for all parameters except for days to 50% emergency and capsule length. Obsa variety with application of 150kg NPS/ha fertilizer gave the highest grain yield (813.9kg/ha). Generally, sesame yield can be increased substantially by applying NPS fertilizer rate up to 150kg/ha to all the three varieties. The application of 150kg NPS/ha specifically to Obsa variety increased grain yield and yield components of sesame through improving soil nutrient availability resulting in better vegetative growth, yield components and, thereby, higher yield and net benefit, 20572.5 ETB/ha thus, can be recommended for the production of sesame in the study area. However, since the study was conducted only at one location for single cropping season and it should be repeated at diverse locations and years to give a conclusive recommendation.
Abstract: In Ethiopia, sesame is the leading oil crop followed by Niger seed and linseed; and oil seed is the third largest sector after cereal and pulse. Production and productivity of sesame is greatly affected by lack of optimum fertilizer rate, inappropriate sowing time, lack of optimum plant population, biotic and a biotic factor such as disease and pes...
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Land Suitability Evaluation for Moringa oleifera Tree Using GIS and AHP Techniques in Weyieb Sub Basin, Ethiopia
Getachew Haile Wondimu,
Woubalem Abera Ayansa
Issue:
Volume 10, Issue 3, June 2022
Pages:
106-118
Received:
13 March 2022
Accepted:
28 April 2022
Published:
12 May 2022
DOI:
10.11648/j.jps.20221003.14
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Abstract: Moringa has been widely used for centuries due to its medicinal properties, nutritional (healthy diet), environmental protection, animal feed and for the purification of turbid surface water. This study examines and evaluate land unit of Weyieb sub basin for the cultivation of moringa tree based on factors that significantly influences the tree growth and productivity. Land suitability analysis is a basic premise for allocating specific land for specific purpose. The study used integrate Geographic Information System (GIS), Remote Sensing (RS) and Analytical Hierarchical Process (AHP) model and weight function to assign suitability weights to criteria and sub-criteria that affect the plant's growth and a predictive cultivation suitability map. Climatic, topographic, edaphic and land use land cover variables were considered in the model as a significantly determinant of moringa tree growth factors. Each of criteria/factor layers were classified (not suitable, less suitable, suitable and highly suitable) based on revied literature and expert level judgement. The Analytical Hierarchical Process indicated that the most influential variable determining Moringa oleifera cultivation were, Elevation, mean annual temperature, mean annual rainfall, soil pH, soil texture, land use land cover and slope, respectively with 8% consistency index. The model results showed that approximately 15.46% (168,001.87 ha) of sub-basin area has optimal growth conditions, 72.75% (790,395.86 ha) suitable conditions and 11.79% (128,042.27 ha) less suitable conditions for cultivating Morina oleifera. The results also reveal that the application and use of integrated GIS and RS with AHP model with weight function is useful for identification and evaluation of land units for M. oleifera cultivation for maximum production output. The results of this study can be useful information for the land-use policy makers and farmers for informed decision-making process regarding the cultivation of M. oleifera in Weyieb sub-basin and other watershed area.
Abstract: Moringa has been widely used for centuries due to its medicinal properties, nutritional (healthy diet), environmental protection, animal feed and for the purification of turbid surface water. This study examines and evaluate land unit of Weyieb sub basin for the cultivation of moringa tree based on factors that significantly influences the tree gro...
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Biodiversity and Ethnobotanical Use Values of Some Plant Species in Gaya Department (Niger)
Oumarou Manirou,
Ali Ado,
Amani Abdou,
Mahamane Ali,
Saadou Mahamane
Issue:
Volume 10, Issue 3, June 2022
Pages:
119-129
Received:
10 June 2022
Accepted:
24 June 2022
Published:
30 June 2022
DOI:
10.11648/j.jps.20221003.15
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Abstract: The objective of this work is to identify in Gaya the plant species socio-economic inter est for the population, their forms of use and the use values of each species used in the different categories of use. Thus, an ethnobotanical survey using an individual interview guide was conducted in 15 villages. In each village, a village assembly was held to identify the target people. A total of 150 people were interviewed individually. In fact, 87 species divided into 67 genera belonging to 33 families were identified. These species are used in six (6) categories of use, namely human nutrition, pharmacopoeia, fodder, firewood, service wood and craft. The result shows a predominance of woody species (64) in the diversity of species and uses. Total use values vary by species and by use category: Vitellaria paradoxa (2.24), Balanites aegyptiaca (2.0), Combretum nigricans (1.42), Borassus aethiopum (1.41), Vitex doniana (1.35), Diospyros mespiliformis (1.32), Prosopis africana (1.21), Sclerocarya birrea (1.20), Tamarindus indica (1.17), Vetiveria nigritana (1.02). Leaves (33%), fruits (26.13%), wood (18.83%) and bark (13.11%) are the most used organs. The degree of knowledge of their importance is more less homogeneous as shown by the Informant Consensus Factor (ICF) and the Informant Diversity Index.
Abstract: The objective of this work is to identify in Gaya the plant species socio-economic inter est for the population, their forms of use and the use values of each species used in the different categories of use. Thus, an ethnobotanical survey using an individual interview guide was conducted in 15 villages. In each village, a village assembly was held ...
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