Volume 2, Issue 6-1, December 2014, Page: 1-4
Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa
Bachir Raho Ghalem, Department of Biology, University of Mascara, Mascara, Algeria
Received: Jul. 31, 2014;       Accepted: Jan. 16, 2015;       Published: Mar. 14, 2015
DOI: 10.11648/j.jps.s.2014020601.11      View  4705      Downloads  190
This study was carried out to investigate the potential use of Artemisia arborescens as a source of antimicrobial agents against pathogens. Essential oils of A. arborescens were collected by hydrodistillation. The antibacterial properties of A. arborescens essential oil was studied on the standard gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and Staphylococcus aureus (gram-positive bacteria), then agar disk diffusion, minimal inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were detected. The results of agar disk diffusion tests showed the inhibition zones as follow: S. aureus 00-18 mm, E. coli 00-16 mm and 08-14 mm for P. aeruginosa. However, their antibacterial activities were lower than those of Gentamicin. The MIC for S. aureus and P. aeruginosa was between 33 and 66 mg/ml, and for Gram-negative bacteria of E. coli was between 66 and 132 mg/ml, while the MBC values of this oil against the tested bacterial strains were between 132 and 264 mg/ml.
Artemisia arborescens Essential Oil, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Antimicrobial Activity
To cite this article
Bachir Raho Ghalem, Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, Journal of Plant Sciences. Special Issue: Pharmacological and Biological Investigation of Medicinal Plants. Vol. 2, No. 6-1, 2014, pp. 1-4. doi: 10.11648/j.jps.s.2014020601.11
K.H.C. Baser and F. Demirci, Essential oils, Kirk-Othmer Encyclopedia of chemical Technology, 4th ed., Sussex: Wiley, 2011.
K. Bremer and C.J. Humphries,“Generic monograph of the Asteraceae-Anthemideae. Bull. Brit. Mus. Lond. Bot,” 1993, 23, p. 71–177.
K. Kubitzki, The families and genera of vascular plants. Vol VIII Flowering plants. Eudicots. Asterales. J.W. Kadereit and C. Jeffrey (Eds.), Springer-Verlag: Berlin Heidelberg, 2007, p.358.
Haider SZ, Mohan M and Andola HC. “Constituents of Artemisia indica Willd. from Uttarakhand Himalaya: A source of davanone,” Phcog Res 2014; 6: 257-9.
J. Yashphe, I. Feuerstein, S. Barel and R. Segal, “The antibacterial and antispasmodic activity of Artemisia herba-alba Asso. II. Examination of essential oils from various chemotypes,” Int. J. Crude. Drug Res, 1987, 25, p. 89–96.
N. Li, Y. Mao, C. Deng and X. Zhang, “Separation and identification of volatile constituents in Artemisia argyi flowers by GC-MS with SPME and steam distillation,” J Chromatogr Sci, 2008, 46(5), p. 401-5.
M.J. Abad, L.M. Bedoya, L. Apaza and P. Bermejo, “The Artemisia L. Genus: a review of bioactive essential oils. Molecules,” 2012, 17(3), p. 2542-66.
M. Aziz , A. Karim , M. El Ouariachi , A. Bouyanzer , S. Amrani , H. Mekhfi , et al, “Relaxant Effect of Essential Oil of Artemisia herba-alba Asso. on Rodent Jejunum Contractions,” Sci Pharm, 2012, 80(2), p.457-467.
M. Ballero, F. Poli, G. Sacchetti and M.C. Loi, “Ethnobotanical research on the territory of Fluminimaggiore (southwestern Sardinia), Fitoterapia, 2001, 72, p. 788–801.
M. El Beyrouthy, N.A. Apostolides, M. Labaki, F. Cazier, S. Najm and A. Abou Kaïs, “Chemical composition of the essential oil of the Artemisia arborescens L. growing wild in lebanon,” LSJ, 2011, 12(1) p.71-78.
G. Bachir Raho and M. Benali, “Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus,”Asian Pac J Trop Biomed, 2012, 2(9), p. 739–742.
L.M. Lima, B. Babakhani, S.A.H. Boldaji, M. Asadi and R.M. Boldaji, “Essential oils composition and antibacterial activities of Eucalyptus camaldulensis Dehn,” Int. J. Med. Arom. Plants, 2013, 3(2), p. 214-219.
E.S. Baykan, G. Reznicek, S.G. Senol, N.U. Karabay Yavasogul, S. Konyalioglu, A. Ulvi Zeybek, “ Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia,” Turk. J.Biol, 2012, 36, p.75–84.
K. Younes, S. Merghache, N. Djabou, D. Merghache, A. Muselli, B. Tabti and J. Costa, “Chemical composition, antibacterial and antioxidant activities of a new essential oil chemotype of Algerian Artemisia arborescens L,” Afr. J. Pharm. Pharmacol, 2012, 6(42) , p. 2912-2921.
M. Saddi , A. Sanna , F. Cottiglia , L. Chisu , L. Casu , L. Bonsignore , A. De Logu, “ Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells,” Ann Clin Microbiol Antimicrob, 2007, 6(10), 7p.
A. Abderrahim, K. Belhamel, J.C. Chalchat and G. Figuérédo, “Chemical Composition of the Essential Oil from Artemisia arborescens L. Growing Wild in Algeria,” Rec. Nat. Prod, 2010, 4(1), p. 87-90.
M. Militello , L. Settanni , A. Aleo , C. Mammina , G. Moschetti , G.M. Giammanco , et al, “Chemical composition and antibacterial potential of Artemisia arborescens L. essential oil,” Curr Microbiol, 2011, 62(4), p.1274-81.
W. Chen, I. Vermaak and A.Viljoen, “Camphor—A Fumigant during the Black Death and a Coveted Fragrant Wood in Ancient Egypt and Babylon—A Review,” Molecules, 2013, 18, p.5434-5454.
D. Tsiri, K. Graikou, L. Pobłocka-Olech, M. Krauze-Baranowska, C. Spyropoulos and I. Chinou, “Chemosystematic value of the essential oil composition of Thuja species cultivated in Poland-antimicrobial activity,” Molecules, 2009, 14(11), p. 4707-15.
P. Owlia, L. Rasooli and H. Saderi, “Antistreptococcal and Antioxidant Activity of Essential Oil from Matricaria chamomilla L,” Res. J. Biol. Sci, 2007, 2 (2), p.155-160.
A. Ben Arfa, S. Combes, L. Preziosi-Belloy , N. Gontard and P. Chalier, “Antimicrobial activity of carvacrol related to its chemical structure,” Lett Appl Microbiol, 2006, 43(2) , p.149-54.
A. Santiesteban-López , E. Palou and A. López-Malo, “Susceptibility of food-borne bacteria to binary combinations of antimicrobials at selected a(w) and pH,” J Appl Microbiol, 2007, 102(2) , p. 486-97.
S. Cosentino, C.I.G. Tuberoso, B. Pisano, M. Satta, V. Mascia, E. Arzedi, F. Palmas, “In-vitro antimicrobial activity and chemical composition of Sardinian thymus essential oils,” Lett Appl Microbiol, 1999, 29, p. 130–135.
L.M. Leandro, F.S.Vargas, P.C.S. Barbosa, J.K.O. Neves, J.A. Silva and V.F. Veiga-Junior, “Chemistry and Biological Activities of Terpenoids from Copaiba (Copaifera spp.) Oleoresins,” Molecules, 2012, 17(4), p. 3866-3889.
M. Mahboubi and N. Farzin, “Antimicrobial activity of Artemisia sieberi essential oil from central Iran,” Iran J Microbiol, 2009, 1(2), p. 43-48.
P. Rattanachaikunsopon and P. Phumkhachorn, “Synergistic antimicrobial effect of nisin and p-cymene on Salmonella enterica serovar Typhi in vitro and on ready-to-eat food,” Biosci Biotechnol Biochem, 2010, 74(3), p. 520-4.
N. Lattaoui and A. Tantaoui-Elaraki, “Individual and combined oils,” Rivista Italiana EPPOS, 1994,13, p.13–19.
M.Marino, C. Bersani and G. Comi, “Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceace and Compositae,” Int. J. Food Microbiol, 2001, 67, p. 187–195.
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